Fluorescence Microscopy

Scientists employ a variety of imaging technologies to visualize and analyze cells on a microscopic level - to reveal what is imperceptible by the naked eye.
These technologies include the classical optical microscope, electron microscopy and laser scanning microscopy. The latter is capable to deliver images of a much higher resolution and contrast than classical optical microscopes. The trick is basically done by having a laser scanning the probe point by point. The reflected light is recorded by a relatively simple device (although of extremely high sensitivity), a single cell sensor, called photomultiplier (Photomultiplier Tubes = PMT) and then the image is pixel by pixel assembled by a computer / software.
The two-photon laser scanning microscopes even further corroborated the breakthrough in biomedical imaging. The applicability of multi-photon excitation, the optical sectioning capability and the superior contrast of these instruments make them an ideal choice for fluorescence imaging of biological samples.

The images in this gallery have been obtained with a confocal LSM (Laser Scanning Microscope) or by conventional fluorescent microscopy. In some cases, the usual "coding" of RGB (red, green, blue) for each channel has been changed to different colors.